IAJPR

Indo American Journal of Pharmaceutical Research

ISSN NO.: 2231-6876
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NOVEMBER 2019
1

CHARACTERIZATION OF NUTRITIONAL COMPONENTS HYDROPHOBICITY BY HPLC BASED CHROMATOGRAPHIC TECHNIQUE

Twinkle S Rana*, Dr.Gautam Sonara2, Dr. Mahesh Salaraya2
1Shree Dhanvantary Pharmacy College, Kudsad Road, Near Railway Station, Kim (E)-394110.
2C. K. Pithawalla Institute of Pharmaceutical Science and Research, Surat.

This work was aimed at determination of log p values of natural compound of great neutitional value. We have selected resveratrol, curcumin, vitamins such as A, D3, and E for this work. Resveratrol and curcumin have been reported to possess diverse biological activities but have poor bioavailability. As reported in literature vitamins are essential for physiology of the body. The bioavailability of resveratrol, vitamins A, D3, E are said to be low due to hydrophobic nature. In this study hydrophobicity was determined via log p value by employing HPLC methods. The log p value of resveratrol ranged between 0.22 to 0.66, curcumin log p value ranged between 1.83 to 2.08 and for vitamin A the log p value ranged between 6.17 to 6.20. The log p value of vitamin D3, E cannot be determined by HPLC as none of mobile phase tested eluted those molecules within 2 hours. Hence HPLC method is not suitable for those molecules. The true values of log p of resveratrol and curcumin differed from predicted values reported in literature, hence further exploration needed in this regard. 


 


2

IMPACT OF PHARMACIST INTERVENTION ON THE AWARENESS ABOUT MENSTRUAL HYGIENE AND ITS IMPACT ON THE INCIDENCE OF REPRODUCTIVE TRACT INFECTION IN RURAL POPULATION AT ERODE

Dr. D. Krishnarajan*, Bitu Chullikkattil .A, Nikhil .V, S. Ramya, Shivaranjini
JKKMMRF’s AJKKSA College of pharmacy, Komarapalayam, Nammakkal-638183, Tamilnadu, India.
The Tamilnadu Dr.MGR Medical University, Chennai, Tamilnadu.

Background: Menstruation is considered as unclean in the Indian society.Menstrual hygiene is an important aspect in women life.This study aims at assessing the knowledge regarding menstruation and hygienic practices.Unhygienic menstrual practices expose them to RTI.If not treated early it will lead to various disabilities.Good hygienic practices such as use of sanitary pads,clothes and changing it every 6 hours,adequate washing of genital area are required during menstruation. Aims and Objectives: To assess the awareness of menstrual hygiene and to improve the hygienic practices among the women in rural population and to determine the prevalence of related disorder. Methodology: A community based interventional study was conducted between February to August 2018 among 200 women of age group 11-50 years,Data was collected using a self structured questionnaire. Results: Among 200 women the mean age of attaining menarche was 13.27 years.127 females had the habit of changing menstrual product before bed.It was found that 124 women attended school/job during menses.Burning sensation was experienced by 46(23%) females and itching by 58(29%) females.There was higher incidence of using soap as vaginal washers in the population (72.5%).The UTI incidence was reported in 58 women in our study. Conclusion: It is important that women be aware of the importance of maintaining menstrual hygiene to prevent the risk of RTI.The program produced significant changes in the knowledge,beliefs and practices of menstrual hygiene and complications from lack of hygiene. 


 


3

A CASE REPORT ON PLUMMER-VINSON SYNDROME

CH. Hari Prasanna, Sk.RahamThunisa, Dr. Gummalla Pitchaiah*
Department of Pharmacy Practice, QIS College of Pharmacy, Pondur Road,Vengamukkapalem, Ongole, Prakasam District, Andhra Pradesh, India – 523272.

Plummer-Vinson syndrome is defined as a classic triad of dysphagia, iron-deficiency anaemia and upper oesophageal webs. In our case, an 18yr old female patient is presented to hospital with complaints of shortness of breath, fatigue, progressive dysphagia since 2 years, pain while swallowing solid foods and weight loss. She was found to be severe anaemic and endoscopy revealed oesophageal web. Anaemia was corrected with elemental iron and blood transfusions. Therefore, through better understanding of underlying anaemic condition, Plummer-Vinson syndrome can be identified in the earlier stages and can be treated appropriately while minimizing risk factors. 


 


4

A VALIDATED CHIRAL HPLC METHOD FOR THE ENANTIOMERIC PURITY OF TRELAGLIPTIN

K. Durga Malleswar1, B. Venugopala Rao*1, K. Venkateswara Rao1, S.Shylaja2.
1Stereokem Private Limited. Plot No-36/A, IDA, Uppal, Hyderabad, Telangana, India.
2Chaitanya Bharathi Institute of Technology, Gandipet,Hyderabad, Telangana, India.


An accurate,precise,simple and rugged Chiral HPLC method has been developed and validated for the quantitation of (S)-isomer in Trelagliptin. The enantiomeric separation of Trelagliptin was achieved Chiral pak AD-3 (250×4.6mm, 3?m) column. The ratio of Hexane,ethanol and diethyl amine (70:30:0.1)in the mobile phase were optimized to obtain the best separation. UV detection was performed at 275 nm. The method is linear over a range of LOQ – 150 % of (S)-isomer. The mean recovery of (S)-isomer was found to be in the range of 95–105%. The method is simple, rapid, accurate, selective and precise, useful in the quality control of bulk manufacturing.


5

AN OVERREVIEW ON MICROSPHERES PREPARATION AND EVALUATION METHODS

Dr. Sarad Pawar Naik. B*, P. Himabindhu, CH. Madhurameenakshi, S. Samitha Krishna, Sreeja. S, T. Aishwarya
Rao's College of Pharmacy, Nellore, A.P – 524 320.

The concept of targeted drug delivery is designed for attempting toconcentrate the drug inthe target tissues of interest while reducing the relativeconcentration of the medication in the remaining tissues i.e., other than target tissues, so that the drugis localized on the targeted site. Hence, other/surrounding tissues are notaffected by the drug. So, carrier technology offers an intelligent approachfor drug delivery systems by coupling the drug into carrier particles such asmicrospheres, nanoparticles, liposomes, niosomes etc., which modulatesthe release and absorption characteristics of the drugs. Microspheres arecharacteristically free flowing powders consisting of proteins or syntheticpolymers which are biodegradable in nature and ideally having a particlesize less than 200 ?m. It is the reliable means to deliver the drug to thetarget site with specificity, if modified and to maintain the desiredconcentration at the site of interest without untoward effects.Microspheres received much attention not only for prolonged release, butalso for targeting of anticancer drugs to the tumour. In future by combining various otherstrategies, microspheres will find the central place in novel drug delivery systems, particularly indiseased cell sorting, diagnostics, gene & genetic materials, safe, targeted and effective in-vivo delivery and supplements as miniature versions of diseased organ and tissues in thebody. 


 


6

ISOLATION OF KAEMPFEROL DERIVATIVES FROM METHANOLIC EXTRACT OF TRIGONELLA FOENUM-GRAECUM

Madhushree K*, Girija K*, Lakshman K, Deepak .M, Uday Kumar
Department of Pharmacognosy, P.E.S College of Pharmacy, Natural Remedies Private Limited, Bangalore -560050.

Trigonella foenum-graecum is an annual plant in the family Fabaceae. In the present study, methanolic extract of the leaves of the Trigonella foenum-graecum was partitioned using water, butanol and ethyl acetate. Then ethyl acetate soluble fraction was subjected to column chromatography to isolate and purify the phytoconstituents. One compound was isolated, purified and characterized as (Kaempferol 3-o-D-glucosyl (1 2)- (611-O-acetyl)-?-D-galactoside 7-O-?-D-glucoside by using IR, Mass and NMR spectroscopy. 


 


7

PREPARATION, CHARACTERIZATION OF PULSATILE MICROSPHERES DRUG DELIVERY SYSTEMS OF ANTI-HYPERTENSIVE AGENT

Suraj Yadav*, S.D. Pande, N. N. Bobade
Department of Pharmaceutics, Vidyabharati College of Pharmacy, Camp, Amravati, Maharashtra, India.

Aim: The aim of present study was to formulate and evaluate the Quinapril Hydrochloride loaded pulsatile microspheres by solvent evaporation technique. Method: Ethyl cellulose and Eudragit Rs 100 are biocompatible polymer is used as the retardant material. The effects of process conditions such as drug loading, polymer type and solvent type on the characteristics of microspheres were investigated. Results: The prepared microspheres were characterized for their particle size, drug loading and drug release. The in-vitro release studies were carried out in pH 1.2, phosphate buffer at pH 6.8 and pH 7.4. The prepared microspheres were white, free flowing and spherical in shape. The drug-loaded microspheres showed 89.30% of entrapment with particle size 235.2?m and the in-vitro release studies showed that Quinapril Hydrochloride microspheres of 85.14%. Conclusion: The study concluded that, the prepared microspheres formulation showed compliance with chronotherapeutic objective of hypertension. 


 


8

PREFORMULATION STUDIES OF TERBUTALINE SULPHATE WITH POLYMERS USED FOR SUSTAIN RELEASE APPROACH

Vijay Kumar*, Mahesh Kumar Kataria
Seth G.L. Bihani S.D. College of Technical Education, Sri Ganganagar, Rajasthan, India.

The major objective of the preformulation study is to develop the elegant, stable, effective and safe dosage form by establishing kinetic rate profile, compatibility with the other ingredients and establish physico-chemical parameter of the drug. Among these properties drug solubility, drug excipients interaction, polymorphic forms and stability plays important role in preformulation study. The solubility of terbutaline sulphate in different solvents was studied. The calibration curve of the drug was prepared in phosphate buffer pH 7.4 by determining absorbance by UV visible spectrophotometer at 276 nm. The compatibility studies if the terbutaline sulphate with the excipients viz. eudragit RS100 and ethyl cellulose was performed using FTIR. The observed data complies for a sustained release formulation. Ethyl cellulose and Eudragit RS 100 may be used to prepare a matrix system for slow release profile formulations. These polymers have compatibility with terbutaline sulphate. The results provide promising baseline information that both ethyl cellulose and eudragit RS 100 are compatible with terbutaline sulphate and suitable to prepare a matrix formulation for sustained release action.