NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF OPIPRAMOL IN PHARMACEUTICAL DOSAGE FORMS
A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Opipramol in Tablets dosage form. The Zorbax Eclipse XDB-C18 Column with 150 length, 4.6mm diameter, and 5μm particle size, with mobile phase 3.48 gms of Di Potassium hydrogen Orthophosphate (0.02M) in 1000 mL of water and by adjusting the pH to 2.5 with dilute orthophosphoric acid (mobile phase solvent-A) and acetonitrile (mobile phase solvent-B) in a gradient mode of separation was used. The flow rate was 1 mL/min and the effluents were monitored at 257 nm. The retention time was 4.43 min. The detector response was linear in the concentration of 5-60 μg/mL. The respective linear regression equation being Y=273255.34x+101066311. The limit of detection and limit of quantification was 0.05 and 0.15 mcg/mL respectively. The percentage assay of Opipramol was 98.96 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate; this is useful for the routine determination of Opipramol in bulk drug and in its pharmaceutical dosage form. The present study demonstrates the applicability of chromatographic method to develop a new sensitive and robust RP-HPLC method for the determination of opipramol in pharmaceutical dosage forms.