SCREENING OF ANTIBACTERIAL AND PHYTOCHEMICAL ANALYSIS OF LEAF AND LEAF DERIVED CALLUS EXTRACTS OF SEBASTIANIA CHAMAELEA (L.) MUELL. ARG
Reena Ganesan, Kamalanathan Desingu, Ragavendran Chinnasamy and Natarajan Devarajan*
Natural Drug Research Laboratory, Department of Biotechnology, Periyar University,
Salem-636011, Tamilnadu, India.
Medicinal plants are widely used as a potent source for isolation of several drugs and formulations in treatment of many diseases. The present study was aimed to carry out preliminary phytochemical and antibacterial activity of six different solvents extracts from leaf and leaf derived callus of S. chamaelea. The maximum percentage of callus mass was achieved in modified MS medium supplemented with different concentration of 2, 4 – D (2.0, 3.0, 4.0 and 5.0 mg/l). In vitro antibacterial activity of leaf and callus extracts were tested against 12 bacterial cultures by agar well diffusion method. The acetone, methanol and ethyl acetate extracts of leaf and leaf derived callus show maximum inhibitory effect. The preliminary phytochemical analysis reflects the presence of phenolic compounds, carbohydrate, alkaloids, phytosterols, fats and oils, terpenoids. The result highlights among two extracts, leaf extract show negligible activity than callus extracts. The present study conclude that invitro raised plants can be utilized for isolation of antimicrobial drugs than wild plants.