EVALUATION OF ANTICHOLINERGIC PROPERTY OF AMITRIPTYLINE AND PROMETHAZINE ON SALIVARY FLOW SECRETION IN NORMAL HEALTHY HUMAN VOLUNTEERS
Objective: Human saliva mirrors the body’s health and can be collected non-invasively, does not require specialized skill. The Aims were twofold: to evaluate the Anticholinergic Property of Amitriptyline and Promethazine on salivary flow secretion in normal healthy Human Volunteers and to evaluate Anticholinergic property between antidepressant and antihistamine. Method: Salivary samples were collected from 12 non-smoking healthy human volunteers aged between 18 – 45 years, weight more than 50kgs.Baseline evaluation of salivary flow was done by placing four sterile absorbent cotton balls onto the vestibule of the mouth for 5 min which were pre-weighed. The weight of the saliva was determined by subtracting pre weight from post weight, in (ml). The volunteers were crossed over after wash out period of 7 days to the other formulation as per randomisation. Results: Effect of Promethazine (25mg), Amitriptyline (10mg), Amitriptyline (25mg) on saliva secretion in ml at different time interval values are expressed as Mean±SD. Promethazine (25mg), Amitriptyline (10mg), Amitriptyline (25mg), have shown significant anticholinergic activity at 5h (p**< 0.01), 6h (p**<0.01) and at 4h (p***<0.001) respectively when compared to baseline using one way ANOVA followed by student’s’ test. Conclusion: Amitriptyline (25mg) showed most significant decrease in salivary secretion levels (ml) at different time intervals when compared with the corresponding levels after administration of Promethazine (25mg), Amitriptyline (10mg). By this study it is proved that Tricyclic Antidepressants and Antihistamines have significant anticholinergic property.