FABRICATION OF EMBELIN INCORPORATED PHYTOSOMES COMPLEX FOR ASSESSING HEPATOPROTECTIVE POTENTIAL AGAINST ACETAMINOPHEN ELICIT HEPATOTOXICITY IN MALE WISTAR RATS.
Esha Yadav, Janey Alam*, Shiva, Sadaf Arfi, Mohammad Khalid, Pallavi Pal2, Shahanuwaz Ahammad, Sadaf Hashmi
1Axis Institute of Pharmacy, Kanpur-208001. Uttar Pradesh, India.
2Krishna Pharmacy College, Bijnor.
The purpose of this research was to see whether Wistar male rats might be protected against paracetamol-induced liver damage by ingesting Embelin-loaded phytosomes complex (EMBP) (PCM). Multiple pharmacological impacts of embelin have been identified, and this study aimed to reduce the required dose while increasing its bioavailability. The hepatotoxicity of paracetamol in Wistar male rats was reduced by belinostatin-loaded phytosomes complex (EMBP) (PCM). Animals group I were treated with 1% CMC for 8 days. PCM was given orally as a single dosage on the eighth day, after a seven-day treatment regimen of "1% CMC," 1 ml/kg per day of EMBP, 50 mg/kg per day of EMBP, 100 mg/kg per day of EMBP, and 100 mg/kg per day of silymarin. Following 24 hours, the animals were sacrificed and their blood was collected via the retro-orbital plexus while under light anaesthesia. In order to determine the hepatoprotective potential, many biochemical markers were analysed. Rats in group IV had significantly lower levels of ALP, AST, ALT, LDH, saturated fat, Total bilirubin, hepatic mass, and comparative hepatic mass contrasted to rats in group II, and higher levels of final body weight, total protein, and alanine aminotransferase (ALB) contrasted to rats in group II. Both silymarin and EMBP, at 100 mg/kg/day, have hepatoprotective potentials similar to those of the gold standard medication, silybin. The study's findings were supported by the histology analysis. Burmese Embelia ribes contained the active component embelin. We employed an innovative method of drug delivery called a phytosome. Six Embelin-loaded phytosome complicated compositions (EMBP1, EMBP2, EMBP3, EMBP4, EMBP5, and EMBP6) were made by combining soy lecithin and chitosan in different proportions and then utilising the anti-solvent precipitate technique as well as the Rotary evaporation technique. Characterization of the phytosome included (SEM) scanning electron microscopy, in vitro release studies, documented drug release kinetic investigations, particle size estimate, percentage output, as well as entrapment efficiency. Among the six formulas, EMBP5 was the most optimised. EMBP5 has the smallest diameter of 345.45±1.231. The immobilization of embelin inside the phytosome complex distinguish from 64.99±1.546 to 81.78±1.151%. The highest entrapment efficiency, 81.78±1.151%, is achieved using the formation of embelin-loaded phytosome complex (EMBP5). The optimal formulation of embelin-loaded phytosomes (EMBP5) was found to contain phytosomes that were less spherical, had a rough and smooth surface, as well as were somewhat aggregated. The phytosomes' in vitro dissolution research revealed a pattern of release. It was determined that fickian diffusion and first order kinetics were involved in the release of embelin from the complex of phytosomes containing embelin (concentration dependant).According to this study, EMBP has hepatoprotective properties equivalent to those of standard silymarin because it showed similar protective potential against acetaminophen-induced hepatotoxicity in male wistar rats.