IAJPR

Indo American Journal of Pharmaceutical Research

ISSN NO.: 2231-6876
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MAY 2018
1

FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL PATCHES OF PIROXICAM

Dr. Siraj Shaikh N*, Pathan Sharukh Y, G.J Khan, Shaikh Saad, Rehan Deshmukh
Department of Pharmaceutics, Ali-Allana College of Pharmacy Akkalkuwa, Nandurbar, Maharashtra, India.

The main objective of present investigation to formulate and evaluate mucoadhesive buccal patches of Piroxicam, using solvent casting method. HPMC K100 M were used as a mucocoadhesive polymer and PEG 400 used as a plasticizer as well as penetration enhancers. The formulated patches of piroxicam were evaluated for their appearance, weight variation, thickness, folding endurance, surface pH, swelling index, drug content, % elongation, mucoadhesive strength, in vitro drug release, kinetic release study and stability study. Among all formulated batches (S1-S8) of buccl patches batch S6 showing maximum drug release after 8 hours 94.77 % and mucoadhesive strength 10.21±0.35g). The stability study optimized batch S6 doesn’t show any changes with respect to previous evaluation carried out before stability study. It may concluded the mucoadhesive buccal patches of Piroxicam were successfully prepared using HPMC K100 M by solvent casting method, evaluated & it is better alternative to conventional drug delivery for the management of pain and arthititis.

2

OVEREVIEW MUCOADHESIVE MICROSPHERE: A REVIEW

Dr.Shaikh Siraj, Shaikh Saad*, Dr. G.J Khan, Shaikh Farhana, Patel Mohsin & Pathan Shahruk
Department of Pharmaceutics, Ali-Allana College of Pharmacy Akkalkuwa, Nandurbar, Maharashtra, India.

Now a day those dosage form which can control the release rate. The microspheres are producing target drug delivery at specific time limit. Microspheres are making important part in novel drug delivery systems. The microspheres are typical free flowing powders consist of synthetic polymer which are biodegradable in nature and having particle size less than 200 um. The microspheres are made from highly transparent glass can perform as much high quality optical micro cavities or micro resonators. The success of these microspheres is limited having provided intimate contact of the drug delivery system with the absorbing membranes. The mucoadhesive microspheres are prepared by using different polymers like HPMC k-4, Ethyl Cellulose, Eudrajit L-100, chitosan, sodium alginate, calcium chloride, guar gum and xanthun gum etc. The microspheres drug delivery system are benefited in various dosage form like nasal drug delivery system, oral drug delivery system, transdermal drug delivery system, colonic delivery, gastro-intestinal drug delivery system, ophthalmic drug delivery system, gene delivery and intra-tumoral drug delivery system. The microspheres are widely accepted to achieve parentral and oral release. Drugs that are easily absorbed from the gastrointestinal tract (GIT) and have a short half-life are eliminated quickly from the blood circulation, so they require frequent dosing. These review focous on overview of different aspect of mucoadhesive microsphere, different preparation method of microsphere as well as evalution parameter and applications of mucoadhesive microsphere.

3

RATIONAL APPROACH TOWARDS THE ROLE OF KALAWNJI (NIGELLA SATIVA LINN) IN MARZ - E - AKYAS KHUSYATUR REHM (POLYCYSTIC OVARIAN SYNDROME)-A REVIEW

Md Sheeraz*, Naquibul Islam, Abdur Rasheed, Arsheed Iqbal, Shameem Rather, Ruqaya Qayoom, Md Danish
Department of Moalajat, Regional Research Institute of Unani Medicine, Srinagar, J and K., 3. Regional Research Institute of Unani Medicine, Bhadrak,Orissa.

Polycystic Ovarian Syndrome (PCOS) is one of the commonest Endocrine disorders. It usually presents during adolescence with menstrual irregularities and weight gain, and with or without signs of hyperandrogenism such as acne and hirsutism. It often affects several family members and is aggravated by obesity. The incidence varies between 0.5 - 4 %. It is prevalent in young reproductive age group (20 -30%). Polycystic ovary may be seen in about 20% of normal women. In Unani System of Medicine, the disease has not been mentioned under the term of PCOS but the description of the disease has been described vividly by various Unani physicians under the headings of qillate tams (Oligomenorrhoea), ehtebas tams (amenorrhea), uqr (Infertility). The best known treatment of PCOS at present is by using allopathic medicines such as clomiphene citrate, metformin and tamoxifen. All these have mild to severe side effects including hot flushes, anorexia, nausea, metallic taste, flatulence, abdominal pain, mild diarrhoea, tiredness, vertigo and allergic dermatitis. So there is a need for alternative treatment. In order to provide safe and more effective medicine for PCOS, a meticulous attempt has been made in this review study to explore the utility of this age old drug Kalawnji (Nigella Sativa linn) in the management of PCOS.

4

DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING HPLC ASSAY METHOD FOR TACROLIMUS IN SEMI-SOLID DOSAGE FORM & BULK DRUG.

Santosh Gejage*, Purnima Amin
Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Elite Status, Nathalal Parekh Marg, Matunga, Mumbai 400019, India.

In the present study we report the development and validation of reversed-phase high performance liquid chromatographic method for the determination of the tacrolimus in presence of pharmaceutical excipients. The developed method was validated with the guidelines of ICH parameters. Also, the forced degradation studies were performed to develop a stability-indicating high performance liquid chromatographic (HPLC) method for tacrolimus-in the presence of the degradation products. The mobile phase was acetonitrile–water 85:15 (v/v). The calibration plot for the drug was linear in the range 25 – 250 ?g/mL was developed on JASCO fully automated HPLC system with Photo-diode array detector at 210 nm wavelength. The method was accurate and precise with limits of detection and quantitation of 4.86 and 14.73 ?g, respectively. Mean recovery was 101.05%. In conclusion we report here an simple, precise and accurate developed RP- HPLC method having validated ICH parameters which can scale up to commercial level for the simultaneous quantification of Tacrolimus in dosage form as well as bulk drugs for quality control purpose.

5

PHASE TRANSFER CATALYSIS: A GREEN METHODOLOGY FOR NEW DRUG DISCOVERY RESEARCH: A REVIEW

Rakesh Devidas Amrutkar
Mahatma Gandhi Vidyamandir’s, Samajshree Prashantdada Hiray College of Pharmacy, Loknete Vyankatrao Hiray Marg, Malegaon Camp, Malegaon-423105, (Nasik), Maharashtra, India.

The major advances in Green Chemistry, especially Industrial Green Chemistry, over the past decade have been in the area of catalysts. One of such techniques is Phase Transfer Catalysis. The use of a Phase Transfer Catalyst (PTC), which Permits or accelerates the reaction between chemical species situated in different phases to overcome the various problems encountered in synthesis. The reaction in organic synthesis can be achieved under solid –liquid PTC and microwaves irradiation in the absence of solvent, generally under normal pressure in open vessel. Therefore, the review presents a brief introduction to PTC and PTC in the generation of compound libraries of over last few years.

6

DESIGN AND DEVELOPMENT OF MUCOADHESIVE BUCCAL TABLETS CONTAINING ANTIDIABETIC ACTIVITY – METFORMIN HCl

A. Deevan Paul, P. Samatha, S. Manasa, R. Munemma, K. Ruchitha, Kommanaboyina. Srinivasa Rao
SVU College of Pharmaceutical Sciences, SV University, Tirupati – 517502.

Mucoadhesive tablets is the preferable route of drug delivery due to the ease of administration, patience compliance, and flexibility in formulation. Every patient would always like to have an ideal drug delivery system possessing the two main properties that are a single dose or less frequent dosing for the whole duration of treatment and the dosage form must release the active drug directly at the site of action. The most frequent polymers used are those derived from HPMC 15M, HPMC 100M and HPMC 4M. The results of Swelling index, mucoadhesive strength and in vitro disintegrating time (sec) ranged from 103.12 to 93.31, 12.11 to 18.16 and 5.02 to 6.15 min respectively. The In vitro dissolution profile indicated faster and maximum drug release from the formulation F4. For the F4 formulation diffusion exponent n value is in between 0.45 to 0.89 so they are following non fickian anomalous diffusion model. Among other results, this article demonstrates that this technology depends on a detailed analysis between the polymer, the physical characteristics of the tablet, the physicochemical characteristics of the drug to be incorporated and the buccal region in which it will remain in contact.

7

ASSESSMENT OF SELF-MEDICATION PRACTICE AND DRUG STORAGE IN DUBER TOWN, OROMIA UNIQUE ZONE, OROMIA REGION, CENTRAL ETHIOPIA

Sintayehu Alemu, Andualem Tesfaye*
School of Pharmacy, Faculty of Health Sciences, Jimma University, Jimma, Ethiopia.

A number of individuals in developing countries do not seek health service for their illnesses; instead they commonly use self-medication. Self-medication could be using drugs existing in home like over the counter drugs, traditional medicine, prescription only drug. Inappropriate storage and use of medicines at home could have a direct influence on public health, the environment and the health-care services and it increases the risk of self-medication. A community based cross-sectional study was conducted in Duber Town from February 9/2016-20/2016.Data was collected by semi structure-questionnaire consisting questions on general demographic, socio-economic as well as on perceived illness/ symptoms in the past one month and actions taken for it. The data collected was screened before it is analyzed. A total 333 respondents interviewed, 120 (36%) had reported different symptoms of illness.45 (37.5%) of the respondents reported with illness practiced self-medication. The most frequently class of drugs for self-medication were analgesics/antipyretics and antimicrobial which account 24(38.1%) each followed by anti-helminthes 6(9.5%).57(17.1%) reported drug storing practice in the house. The most commonly stored drugs are analgesics/antipyretics (48.5%) followed by antimicrobial (45.5%).There is high prevalence of self-medication practiced and Analgesic/antipyretics, antibiotics and anti-helminthes were identified commonly used drugs for self-medication. Moreover Community practiced drugs storage in the house and it is important to provide health education on risk and benefit of self-medication and drug storage.

8

ANTI-CANCER AND ANTI-INFLAMMATORY ACTIVITY OF SALVADORA PERSICA L.

Baba Fakruddin K1, Ranganayakulu G S1, Subramanyam P2, Muralidhara Rao D3*
1Department of Biotechnology & Botany, Rayalaseema University, Kurnool-518007, INDIA.
2Department of Botany, Loyola Degree College (YSRR), Pulivendula-516390, INDIA.
3Department of Biotechnology, Sri Krishnadevaraya University, Ananthapuramu-515003, INDIA.

In the present work we studied Salvadora persica L. crud extracts of ethanol, acetone and water extracts of the leaves, stem bark and fruit peels on anti-inflammatory and anti-cancer activity in HeLa cell lines against by trypan blue dye exclusion method. Ethanol fraction of Salvadora persica leaf extract (300 mg/kg b.w. p.o.) has shown significantly high percentage of anticancer property as 69.1% of dead cells. Furthermore the effect of ethanol, acetone and water extracts of leaves, stem bark and fruit peels of Salvadora persica demonstrated that the anti-cancer activity in EAC cell inoculated swiss albino mice. Ethanol extracts of leaf, bark and fruit feels at 300 mg/kg b.w. p.o. dose shown significant prolongation of lifespan, reduction in tumor volume and improvement in the hematological parameters when compared to the other extracts of Salvodara persica. There by it can be concluded that ethanol extracts of leaf, bark and fruit peels possesses better anti-inflammatory and anticancer activity at 300 mg/kg b.w. p.o. dose than acetone and water extracts.

9

DEVELOPMENT AND OPTIMIZATION OF SELF EMULSIFYING DRUG DELIVERY SYSTEM OF BCS CLASS IV DRUG

S.P.Chaudhari, S.C.Daswadkar, A.V.Kulkarni, N. K. Thamke
Dr. D. Y. Patil College of Pharmacy, Akurdi, Pune-411044.

The present study aims to develop Self-emulsifying Drug Delivery System (SEDDS) to increase bioavailability of drug and dissolution characteristics of dosage form. Primarily screening of different oils, surfactants and cosurfactants for solubility of drug was done. Based on solubility study Olive oil (oil) and Tween 80 (surfactant) Labrafil 1944 CS as cosurfactant were selected for further study. Optimization of SEDDS formulation was done by two methods firstly using design expert applying Mixture composite design and secondly by plotting Ternary diagram to find out optimum concentration of the oil and surfactant. Batches of SEDDS were prepared using Olive oil as oil, Tween 80 as surfactant and Labrafil 1944 CS and evaluated for Droplet size, Drug content, phase separation study and % Transmittance, Drug release. From the mixture composite design F3 formulation showed better drug release (100%) and lowest globule size (2.0 um) as compared to other batch of liquid SEDDS. Hence F3 formulation was considered as optimized formulation and was selected for further study. To form more stable and acceptable dosage form of drug, Solid Self Emulsifying Drug Delivery System (S-SEDDS) were prepared. The Development of S-SEDDS was attempted using kaolin and microcrystalline cellulose by Adsorption carrier technique.K3 and M3 were further analysed for in vitro dissolution studies. From dissolution study it was observed that there was an increase in solubility and decreases dissolution rate of Azithromycin dihydrate as compared to pure drug. K3 formulation had shown good dissolution profile and high similarity with marketed formulation than M3 formulation. The optimize formulation was then evaluated for its bioavailability study .The bioavailability studies for SEDDS formulation and marketed formulation were carried out using wistar abino rats. Hence F3 S-SEDDS K3 formulation containing Olive oil, Tween 80, Labrafil M 1944 CS and Kaolin may possibly be used to improve the bioavailability and dissolution characteristics of Azithromycin dihydrate.

10

A PROSPECTIVE OBSERVATIONAL STUDY ON THE EVALUATION OF ANTI-TUBERCULAR THERAPY INDUCED ADVERSE DRUG REACTIONS IN PATIENTS WITH TUBERCULOSIS

Dr. Syeda Zaineb Humaira Hussaini1, Misbah Unnisa2, Syeda Atqiya Ara2, O. Krishna Prasad2, K. Ranadeep Reddy2
1Department of Pharmacy Practice, Bhaskar Pharmacy College, Hyderabad, Telangana, India.
2Pharm.D(Doctor of Pharmacy), Bhaskar Pharmacy College, Hyderabad, Telangana, India.

BACKGROUND: Tuberculosis is a disease of great antiquity and ranks the second highest contagious disease globally which is caused by Mycobacterium Tuberculosis. According to WHO, an adverse drug reaction is defined as “a response to a drug which is noxious, unintended and which occurs at normal doses used for prophylaxis diagnosis or therapy of disease or for the modification of physiological functions”. METHODOLOGY: This study was conducted with the objective to evaluate the anti- tubercular therapy induced adverse drug reactions in patients who were diagnosed with sputum positive tuberculosis in Govt. General and Chest Hospital, Erragadda, Hyderabad. This study is observational in nature and the subjects enrolled under this study were about 150. Informed consent was obtained from all the subjects. Subjects recruited in the study were admitted as in-patients in the hospital. The causality assessment of suspected ADR’S was done by using WHO-UMC SCALE and severity assessment by using modified Hartwig and Siegel scale. RESULTS: This study identifies the incidence and patterns of adverse drug reactions in patients who were prescribed with DOTS Therapy (category I and category II drug regimens). Out of 150 patients, 44 patients were suspected with 53 ADR’S, in which female patients(60.3%) were more prone to the occurrence of ADR’S when compared with male patients(39.7%).Based upon the categories of the drug regimens more number of ADR’S were associated with category II (63%) than ADR’S associated with category I (37%).More number of probable reactions (51%) and moderate reactions (55%) were identified by using WHO-UMC scale and Hartwig and Siegel scale respectively. CONCLUSION: Major adverse reactions in anti-tubercular drugs can cause significant morbidity and compromise treatment regimens for tuberculosis. These events may result in substantial additional costs because of added outpatient visits, investigations and in more serious instances hospitalization. As a result, the risk of treatment failure and relapse are higher.Regular ADR monitoring is required to reduce morbidity and to improve patient compliance after initiation of anti-tubercular therapy. Further study is required for preventing the occurrence of ADR’S.

11

GQSAR, PHARMACOPHORE MAPPING AND MOLECULAR DOCKING STUDIES OF SOME PYRIDAZINONE DERIVATIVES.

Rakesh Panda*, Monica Kachroo, Lokesh Pathak
Department of Pharmaceutical Chemistry, Al-Ameen College of Pharmacy, Bangalore.

Various literatures are reported on the versatility of pyridazinone molecules, an article was selected with reported IC50. 20 compounds (S1-S20) were selected for combined and stepwise in silico studies on pyridazinone moieties in the form of GQSAR, pharmacophore matching and molecular docking studies. These studies revealed important physicochemical properties of pyridazinones responsible for various biological activities. Group based QSAR (GQSAR) on COX-2 was carried out and 2 best models were studied. Pharm Mapper webserver was used for in silico reverse target screening to find out potential therapeutic targets for pyridazinones. The targets identified using in-silico method like Tyrosine phosphatases (PDB ID- 1NWL), Sorbitol dehydrogenase (PDB ID- 1PL6), Cell division protein kinase 2 (PDB ID - 2B54), Mitogen activated protein kinase 14 (PDB ID-2ZB1), Glucocorticoid receptor (PDB ID- 3CLD) were further screened by molecular docking approach to find best target among these. Docking scores and interactions of pyridazinones revealed Mitogen activated protein kinase 14 as its putative target. MAP 14 kinase is already a well known anti- inflammatory mediator receptor protein hence this in silico study and the data generated have proved that the molecules having pyridazinone moiety could be developed as anti-inflammatory lead compounds.

12

PROTEOMIC STUDIES OF CHEMICAL CONSTITUENTS OF COMMELINA COMMUNIS

Nagavalli Dhandapani*, Saranya Sundaralingama, Anu Baskaranb, Aruna Muthusamyb Brindha Vasudevanb, Dhivya Muruganb, Divya Gopalakrishnanb, Sujarithajayarajb
Pharmaceutical Chemistry, Adhiparasakthi College of Pharmacy, Melmaruvathur.

The present research work was aimed to discover anti-viral and anti-dengue activity of the phytochemical constituents present in Commelina communis using Molecular docking studies. In this research work, we have screened 10 chemical constituents from Commelina communis through docking studies, in an effort to develop novel anti-dengue and anti-viral activity, the target enzymes were chosen for the study is 4M9K, 3P54, 2V33, 5FC8 and 5B1C using Argus lab 4.0.1 software. Among the 10 screened compounds of Commelina communis, shows best to moderate (-9.49194kcal/mol to –5.61913kcal/mol) binding energy and various bonding interactions against the targeted proteins. On the basis of docking study results, it can be concluded that chemical constituents of Commelina communis, serve as promising chemical probes to design therapeutic agents with anti-dengue, and antiviral properties.

13

DEVELOPMENT AND CHARACTERIZATION OF SOLID SELF EMULSIFYING DRUG DELIVERY SYSTEM FOR PROSTATE CANCER

Mr.Sunil T. Galatage*, Dr. S. M. Patil, Dr.R.M.Chimkode
Sant Gajanan Maharaj College of Pharmacy Mahagaon, Tal-Gadahinglaj, Dist-Kolhapur-416502, Maharashtra.

Present study was focused on improving the therapeutic performance of fosfestrol. It has poor aqueous solubility which turn in to reduced oral bioavailability which shows less therapeutic effectiveness in treatment of prostate cancer. Further oral administration of fosfestrol is associated with severe GIT irritation. This results in a compromised oral use of fosfestrol. To solve these problems associated with fosfestrol, we have formulated the self emulsifying drug delivery system (SEDDS) for fosfesrol and evaluated for droplet size, solubility study, ternary phase diagram study, self emulsification rate study, cloud point determination, freeze thaw cycle, surface morphology by SEM, zeta potential, In-vitro drug release study. The prepared SEDDS of fosfestrol was found to be satisfactory and drug content was found to be 82-99%, droplet size was in the range of 4 to 13 ?m, self emulsification time was in the range of 54 to 160 sec. and maximum cloud point and drug release was found to be for FO9 was 700C and 97.3% respectively. Drug release of the drug follows zero order kinetic model. The developed formulations have shown improved dissolution profile in comparison to marketed formulation and pure drug. The developed solid SEDDS is having potential to improve the therapeutic potential of anticancer drug fosfestrol in the treatment of advanced prostate cancer and it was proved by anticancer activity of SEDDS formulation FO9 on prostate carcinoma cells.

14

THE HEN'S EGG TEST - CHORIO-ALLANTOIC-MEMBRANE (HET -CAM) AND HAEMOLYSIS STUDY AS AN ALTERNATIVE TEST FOR OCCULAR IRRITANCY ANIMAL EXPERIMENTS (Draize Test)

Deepak G Wagh, Dr. Sadhana R Shahi
Department of pharmaceutics, Yash Institute of Pharmacy, South city, Opp A.S Club, Waluj Road, Aurangabad, Maharashtra, India.431136.

Human eyes are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular irritancy for their safe handling and use before release into the market. Draize rabbit eye test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) Hen Egg Test-Chorio-Allantoic-Membrane (HET-CAM) test (ii) RBC Haemolysis study.

15

FORMULATION AND EVALUTION OF GASTRORETENTIVE MUCOADHESIVE TABLETS OF ANTIRETROVIRAL DRUG

Mohammad Bakhatwar*, Chikkala Siva Ramesh
Aditya College of Pharmacy, ABD Road, Surampalem, East Godavari.

Objective: The aim in the present investigation was to design and evaluate the gastro retentive mucoadhesive delivery system of Zidovudine with a view to enhance the gastric residence time and its oral bioavailability.In the current work, direct compression method has been employed to prepare GRDDS of Zidovudine with HPMC K100M, NaCMC, Carbopol934P and PVP K30 with different drug to polymer ratios. Various evaluation tests and stability tests were conducted.Pre formulation studies for all formulations showed satisfactory results. FTIR studies evidenced that drugs and polymers were compatible. Followed by The formulated tablets were subjected for various evaluation parameters like hardness(3.98±0.654 to 3.09±0.455 kg/cm2), thickness(2.98 ±0.13-2.29 ±0.09 mm), weight variation(303.69 ±1.428-298.35 ±1.006 mg), drug content(100.65 ±0.45-97.81 ±0.590 mg),friability(0.75 ±0.04-0.45 ±0.07), results showed all extensively passed, all the evaluation tests as per USP standards. Swelling index, mucoadhesive strength, Ex-Vivo Residence Time results were propositional to concentration polymers. In dissolution study it was observed that release of Zidovudine from different formulations was spread over a period of 8-12hrs depending on the polymer’s concentration and viscosity. mong rio s orm l tions st died 1 ont ining 1 nd sho ed dr g rele se t the end o 12hrs nd o nd to e st le t C and 75% RH following a three month stability study.

16

FORMULATION AND EVALUATION OF BIOADHESIVE BUCCAL DRUG DELIVERY OF LESINURAD TABLETS

Suresh Kumar.P*, Bhavya.S, Sindhu.V, Bindhu.T.
Department of Pharmaceutics, Browns College of Pharmacy, Khammam, India.

The purpose of this research was to formulate and evaluate bioadhesive buccal tablets of Lesinurad using sodium alginate, sodium carboxy methyl cellulose and carbopol 934. The tablets were evaluated for weight variation, thickness, hardness, friability, surface pH, mucoadhesive strength, swelling index, in vitro drug release, ex vivo mucoadhesion time and ex vivo drug permeation. Tablets containing sodium alginate as a polymer (F2) had the maximum percentage of in vitro drug release. The surface pH of all tablets was found to be satisfactory (between 6.5 and 7.43), close to neutral pH; hence buccal cavity irritation should not occur with these tablets. F2 batch was considered optimum based on good bioadhesive strength (02.60±0.03 gm) .The drug release from optimum batch followed zero order kinetics with Higuchi release. Drug excipients compatibility study showed no interaction between drug and excipients. Stability study of optimized formulation showed that tablets were stable at accelerated environment condition. Thus, buccal adhesive tablet of Lesinurad could be an alternative route to bypass hepatic first pass metabolism and to improve bioavailability of Lesinurad.

17

PREPARATION AND EVALUATION OF HERBAL FACE PACK

Bhawana Bhatt*, Preeti kothiyal, Sudhakar Kaushik, Soniya Badoni
Shri Guru Ram Rai University, Dehradun, Uttarakhand, India.

The objective of this work is to formulate and evaluate a cosmetic herbal face pack for all type skin by using natural ingredients with the varying concentrations, Three different formulations containing ingredients such as Mint , Methi , Green tea, multani mitti, White sandal wood, orange peel procured from the local market were prepared named as F1 , F2 & F3, then passed through sieve no 44 mixed geometrically and evaluated for its organoleptic and physio-chemical, general powder and chemical evaluation. The dried powder of combined form had passable flow property which is suitable for a face pack. All prepared formulations were evaluated by different parameters like organoleptic properties and physio-chemical parameters and stability along with irritancy test & photo irritation. Herbal face packs or masks are used to stimulate blood circulation, rejuvenates the muscles and help to maintain the elasticity of the skin and remove dirt from skin pores. In this study it is concluded that all the formulations of face packs found to be good in physical parameters, free from skin irritations so we found good properties for the face packs and further optimization studies are required on this study to find the useful benefits of face packs on human use as cosmetic product.

18

TRANSDERMAL DRUG DELIVERY SYSTEM AN OVERVIEW

Mr. Arjun Bhamare, Mr. Rajendra Surawase, Mr. Dattatraya Shinkar Mr. Yashpal More
Loknete. Dr. J. D. Pawar College of pharmacy Manur (Kalwan) Tal. kalwan Dist. Nashik.

The conventional oral dosage forms have some drawbacks like poor bioavailability, first pass metabolism, of drug, frequent dosing which may leads to patient non compliance. The transdermal drug delivery system is one of the type novel drug delivery system which overcome the problems arises from conventional dosage forms. A transdermal patch is medicated adhesive patch these is place on the surface of skin and these deliver a specific dose of drug medicament through the skin and in to bloodstream. It also helps to healing to a mild injured surface of skin. An advantage of a transdermal drug delivery route over other types of medication delivery such as oral, topical, intravenous, intramuscular, etc. is that the patch provides a controlled release of the medication into the patient and it gives painless drug delivery to the patient over intravenous route of drug administration. Its having some disadvantage it permit only drug having smaller molecular size. This review gives valuable information about the TDDS like its advantages, disadvantages, types of TDDS, different methods for formulation of transdermal patches, different evaluation of transdermal patches. Different methods for preparation of transdermal patches. Skin permeation enhancement techniques have been developed to improve the bioavailability of drug.

19

SPR BIOSENSOR FOR EARLY AND RAPID DETECTION OF PROCALCITONIN, D-DIMER AND VITAMIN-D FROM HUMAN PLASMA / SERUM BY USING MINI VIDAS SYSTEM

Yuvaraj sampathkumar1*, M.Dinakaran1, N. Silambarashi2, S.Elumalai3

1Department of Clinical laboratory, Chennai National Hospital, 12A, Jaffer Syrang Street, Chennai - 600001.

1Department of Cardiology, Chennai National Hospital, 12A, Jaffer Syrang Street, Chennai - 600001.

2Department of Microbiology, Vinayaka Mission’s Kirupananda Variyar Medical College & Hospitals, Salem.

3Department of Biotechnology, University of Madras, Guindy Campus, Chennai - 600 025.

 

The biomerieux minividas chemiluminescence immunoassay is a modernized, reliable, and unbeatable technique which is used to quantify the Procalcitonin, D-dimer and Vitamin-D from the human serum sample. For most of the patient’s symptoms are non specific. The immunoassay (RIA) methods results are showing the significant inconsistency, lead to miscalculation in the immunoassay methods when compared with the reference method. LIA, ELFA, ECLIA and SRP immunosorbent assay methods are highly effective, compared with qualitative immunoassay. Hence, the SPR biosensor is a high sensitivity pipetting device, applied to recognize the sepsis, severe sepsis, thrombosis (DIC, DVT and pulmonary embolism), rickets, deficiency or insufficiency of vitamin D.

20

EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF GUAZUMA TOMENTOSA L. LEAVES IN WISTER ALBINO RAT.

Mr. C. Yogesh*, Mr. A. Imtiyaz, Dr. K.Vanita, Dr. R. Sudha
Oriental College of Pharmacy, Sector 2, Behind Sanpada Railway Station, Sanpada West, Navi Mumbai, Maharashtra 400705.

Guazuma Tomentosa.L a member of the Sterculiaceae family, is used in folk medicine because of its antimicrobial, antioxidant, and antihypertensive properties. Most of the research work carried out on this plant has focused on the leaves because of its high concentration of antioxidant, kaempferol, proanthocyanidins. The leaves and flowers of Guazuma tomentosa, though less studied, are also used as a remedy for different conditions, such as inflammation, kidney and gastrointestinal diseases, fever and diabetes. The aim of this study was to assess the anti-inflammatory effects of the ethanolic extract from leaves of Guazuma tomentosa in a model of carrageenan paw edema and leukocyte migration method by carrageenan as inflammatory agent, using the indomethacin as a reference. Therefore, the extract was administered single dose orally to three groups of Wistar rats at doses of 500, 250 and 125 mg/kg, with a indomethacin (10 mg/kg) was given of the extract. Pretreatment with Guazuma Tomentosa or indomethacin decreased the inflammation area in a dose-dependent way. In the present study, leaves extract of Guazuma tomentosa L., were studies for anti-inflammatory activity by in-vivo method. The extracts were found to possess significant activity at all selected doses i.e. 125, 250 and 500 mg/kg body weight, Guazuma tomentosa L. showed comparable results with the standard drug. It showed maximum percentage inhibition of 53.25%, which were comparable with the Standard, indomethacin, which showed 51.08% inhibition. In leukocytes migration method by exudate volume standard shows 56.96% inhibition, while 125, 250, and 500 mg/kg ethanolic extract of Guazuma tomentosa L. The probable mechanism for the acute anti-inflammatory action could be due to anti-bradykinin activity and inhibition of synthesis of prostaglandins and other inflammatory mediators like histamine and serotonin in early hours of inflammation.

21

DEVELOPMENT AND METHOD VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION AND FORCED DEGRADATION OF CEFOPERAZONE AND TAZOBACTAM IN BULK AND PHARMACEUTICAL DOSAGE FORM

Ansari Yunus N1*, Patil Vikas V2, Puri Abhijeet V1.
1St. John Institute of Pharmacy and Research, Palghar (MS) India.
2KVPS Institute of Pharmaceutical Education Boradi, Shirpur, (MS) India.

Objective: A new precise, accurate, sensitive and robust reverse phase high performance liquid chromatography (RP-HPLC) method was developed for Cefoperazone and Tazobactam in marketed preparation. Method: The Chromatographic separation was achieved on Thermo BDS Hypersil C18 column (250 × 4.6 mm i.d.5 ?) at ambient temperature. A Binary mobile phase consisting of 10 mM potassium dihydrogen phosphate buffer, pH 2.8 and Acetonitrile (80:20, v/v) was delivered through a column at a flow rate of 1 ml/min. Estimation was performed at a coveted wavelength of 254 nm. Result: The method was linear over the concentration range of 40-200 ?g/ml and correlation coefficient (r2) was found to be 0.9997 for Cefoperazone (CEFO) and 5-25 ?g/ml for Tazobactam (TAZO), correlation coefficient was found to be 0.9996. The retention times of the CEFO and TAZO were found to be 3.667 min and 4.737 min. respectively. The percentage content for Cefoperazone 98.94±1.0 and of Tazobactam was 99.69±0.96 in the marketed formulation. The low value of % Relative Standard Deviation (%RSD) was 0.224 and 0.018 for Cefoperazone and Tazobactam, respectively, indicates the reproducibility of this method. The low value of Limit of detection (LOD) and Limit of quantitation (LOQ) suggests the sensitiveness of method. Conclusion: It can be concluded from the outcomes that the proposed RP-HPLC method was found to be simple, basic, accurate, robust and precise for the normal analysis of Cefoperazone and Tazobactam in bulk and sterile dried injection dosage forms. This method was validated as per ICH guidelines.

22

REVIEW ON PUMPKIN SEED OIL (C. MAXIMA, C.PEPO)

E.Susithra, Paul Joplong Nongmin, P.Swathi, J.Magibalan, V.Balaji
Department of Pharmacognosy, School of pharmaceutical Sciences, Vels Institute of Science and Technology (VISTAS) Pallavaram, Chennai-600117, TamilNadu, India.

The seeds of these fruits has high source of bioactive constituents which can be used as an eatable oil and can be also be used in the pharmaceutical industries for nutraceutical. It was found to contain high amount of free fatty acids like linoleic, palmitic and stearic with their relative distribution of 43.8%, 33.1%, 13.4% and 7.8% respectively, which represents of about 98 + 0.1% of the total fatty acids amount. The dominant content of tocopherol are the ?-tocopherol where it is being predominant over ? -and ?- tocopherol. The antioxidant activities of the pepo seed oil indicates the good cytoprotective with the modification of few chemical compounds. The usefulness of the pepo seed oil in the management of benign prostrate may therefore become as a good medicinal product in the direct inhibition of the prostrate growth. With the investigation of hypercholesterolemia patients has been found out that it can reduce the level of total and LDL cholesterol levels by 30 – 50%. Pretreatment of the animals by PSO, then administration of the same doses of FEL produce more reduction in the arterial blood pressure ranged from 17.3"1.75 to 40.9"1.35 mmHg.

23

DEVELOPMENT AND EVALUATION OF BOSENTAN PELLETS FOR PROLONGED DRUG RELEASE

Narender Karra*, Narayana Raju P, Sivakumar R
Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad, Telangana, India.
Geetanjali College of Pharmacy, Cheeryal, Medchal Dist, Telangana, India.

The present study was focused on preparation and evaluation of SR pellets of Bosentan. The preparation of extended release (ER), drug pellets of Bosentan were prepared by using fluid bed coating (FBC). These drug-loaded pellets were further coated with ethyl cellulose of two viscosity grades and Eudragit as rate controlling polymers, hypromellose as pore former and binder, acetyl tributyl citrate as plasticizer, and magnesium stearate as anti-adhering agent. The prepared pellets were evaluated for drug content, invitro dissolution, DSC, FTIR and SEM. The prepared pellets were subjected for accelerated stability study. The drug release was extended up to 24 hours and the dreg release was mainly depends on the polymer type and polymer proportion. Accelerated stability showed good similarity with the initial formulation indicated good stability for 6 months. DSC and FTIR study showed the no drug polymer interaction. This technique can be showed good commercial success.