EVALUATION OF ANTICANCER POTENTIAL OF IPOMOEA RENIFORMIS CHOIS (CONVOLVULACEAE) LEAVES AGAINST EHRLICH ASCITES INDUCED CANCER IN MICE
The present study was carried out to evaluate the antioxidant and antitumor activity of methanolic and aqueous extract of leaves of Ipomoea reniformis has (IPR) against Ehrlich ascites carcinoma (EAC) in mice. The antioxidant activity of Alcoholic and Aqueous extract of leaf of Ipomoea reniformis were carried out by DPPH and lipid per oxidation anti-oxidant assay. It is comparatively studied on the basis of EC50 value with reference to EC50 value of ascorbic acid. The EC50 value of standard, Alcoholic and Aqueous extract of Ipomoea reniformis were found 2.278, 2.392, 2.951 and 66.45, 68.11, 100 by DPPH and lipid per oxidation anti-oxidant assay respectively. For the anticancer potential of Ipomoea reniformis chois. (Convolvulaceae) leaves evaluated against Ehrlich Ascites Carcinoma induced cancer in mice. The EAC cells were maintained in Swiss albino mice, by intraperitoneal (i.p.) transplantation in every 9 days. The ascetic fluid was collected by syringe and the tumour cell count was performed in a neubauer chamber (haemocytometer) and 2 x 106 cells/per ml was obtained by dilution with normal saline. EAC cells of 9 days old were used for the screening study.72 Swiss albino mice were divided into six groups (n = 12) mentioned food and water ad libitum. All the animals in each groups except Group-I, received EAC cells (2×106 cells/mouse i.p.). This was taken as day ‘0’. Group-I served as normal saline control (5 ml/kg i.p.), Group-II served as EAC control. 24-h after EAC transplantation, Group-III received reference drug 5-FU (20 mg/kg i.p) for nine consecutive days and Group-IV to Group VI received plant extract by oral rout for nine consecutive days respectively. Twenty-four hours of last dose and 18 h of fasting, 6 animals of each group were sacrificed by cervical dislocation to measure antitumor, hematological and biochemical parameters, the rest were kept with food and water adlbitum to check mean survival time and percentage increase in life span of the tumor host and results of hematological, biochemical and antitumor were studied respectively. All the studied parameter reveals the Alcoholic and Aqueous extract of Ipomoea reniformis having potent antioxidant and anticancer potentials.