Sundaramoorthy M1, Prabaharan C2, Purusothaman B3 and Saravanan T.S4
1PG & Research Department of Zoology, AVC College (Autonomous), Mannampandal, Tamilnadu, India, Pin-609 305.
2Department of Biotechnology, ARJ College of Engineering and Technology, Mannargudi, Tamilnadu, India, Pin-614 001.
3School of Chemical Engineering and Biotechnology, SASTRA University, Thanjavur, Tamilnadu, India, Pin-613 401.
4Department of Biotechnology, Dr. M.G.R Educational and Research Institute University, Chennai, Tamilnadu, India, Pin-600 095.
Antimicrobial peptides (AMPs) play a vital role in innate and adaptive immunity acting as multifunctional molecules in offering in vitro antimicrobial activities, immunomodulation, mediation of inflammation, cell proliferation, migration, wound healing etc., Herein We demonstrate the antibacterial and wound healing properties of certain protein compounds partially purified from the heart tissues of certain selective slaughter house animals (goat, cow and chicken) by chemical methods. The antibacterial proteins (ABPs) were semi purified by consecutive procedures of acid extraction, 30% ammonium sulphate precipitation and dialysis. The yields of respective ABPs were found to be 46.1, 48.3 and 49.4 mg/g wet weight. The proteins isolated from the heart tissues of these three animals were tested for their antibacterial activities by performing Minimum Inhibitory Concentrations (MICs). They exhibited potential antibacterial activities against all the eight test isolates. The MICs of the protein solutions fell in the range of 8-63 mg concentrations. Subsequently their wound healing properties were carried out on rat wound models. These proteins showed 96.7%, 96.9% and 100% of wound healing activities respectively on excision wound models of albino rats at 9th post wounding day. The result was significantly better than the wound models treated with negative and positive controls. The proteins were also characterized for TLC patterns and absorption spectra. The post TLC of each protein sample showed only a single band with Rf value in a narrow range (0.341 to 0.364). The λ-max of the proteins was found to be 280 nm invariably. The results conclude that the semi-purified ABPs from the heart tissues of goat, cow and chicken fulfills the major criteria such as natural occurrence, low molecular weight, antibacterial and wound healing potentials to render themselves as natural antibiotics for the treatment of infectious diseases.