The present research explores the use of eco-friendly silver nanoparticles (AgNPs) created through a green method, employing Acacia Catechu extract, to combat infections in burn wounds. The successful synthesis of AgNPs was confirmed using UV-Visible spectroscopy, with the extract acting as a reducing and stabilizing agent. The AgNPs' size and shape were determined through Scanning Electron Microscopy and Zeta Sizer. These nanoparticles displayed potent antimicrobial properties against a range of bacteria commonly associated with burn wound infections, showing promising results in agar well diffusion assays and minimum inhibitory concentration (MIC) determination. The antimicrobial action involved disrupting microbial cell membranes and generating reactive oxygen species (ROS), leading to oxidative stress within the cells. The green synthesis approach presents a sustainable alternative, avoiding hazardous chemicals, and the natural plant extracts may contribute to wound healing and anti-inflammatory properties, further enhancing the therapeutic effectiveness of the AgNPs. This study underscores the potential of Acacia Catechu-mediated AgNPs as a valuable solution for preventing infections in burn wounds, prompting further investigation into their in vivo effectiveness, wound healing capabilities, and long-term stability. Ultimately, this research concludes that Acacia Catechu-mediated AgNPs hold promise for safer and more effective wound care 

RP-HPLC method for determination of anti-diabetic drug Liraglutide in bulk and tablet formulation has been developed and validated. A RP-HPLC method was performed on Agilent Zorbax Eclipse XDB C18(100mm×4.6mm, 3.5?m) column using PHP Buffer(10mM): MeOH: ACN 60:20:20%V/V (pH 3.8 Adjusted with 0.1% Ortho-phosphoric acid), as a mobile phase at a flow rate of 1.5ml/min and analytes were monitored at 245nm. The retention time for Liraglutide was found to be 2.66min. The peak obtained was symmetrical with tailing factor less than 2 and theoretical plates more than 2000. The linearity was found in the concentration range 10-60?g/ml for Liraglutide. LOD and LOQ was found to be 0.85?g/ml and 2.60?g/ml. for Liraglutide. The percentage mean recovery at three different levels (80%,100%120%) for Liraglutide was found to be 100.4 -105.37%w/w. The percentage assay of Liraglutide in dosage form was found to be 99.1-99.5%w/w. The method was validated in accordance with ICH guidelines Q2 (R1) and was found to be Specific, Accurate, Precise, Robust and can be successfully applied for routine analysis of Liraglutide in bulk and pharmaceutical dosage form. The developed method has been found suitable for routine analysis of Liraglutide in bulk and tablet formulation. 

Aim of the present work is to provide a short communication on role of nutrients in fermentation. Aged food varieties or fermented products are acquiring prominence among customers for their conceivable helpful and high showcasing esteem and their gainful impacts are getting more obvious with aggregating results from clinical studies.After a comprehensive study the information provided in this article presents that in fermentation, nutrients playa beneficial role. 

Aceclofenac-loaded niosomal cream were developed to provide sustained and localised drug delivery into the skin, increasing the medication's anti-inflammatory activity and lowering its systemic adverse effects. Different molar ratios of surfactant, cholesterol, and charge inducers were used to create niosomes using the thin-film hydration method. Formulations were characterized for entrapment efficiency, morphology, size, and zeta potential. In-vitro release and stability studies were conducted on selected formulations. Niosomal cream were evaluated for spreadability, pH, rheological behaviour, and in-vivo anti-inflammatory efficacy. The encapsulation efficiency of the formulations was good, reaching 70%. Vesicles had an entrapment efficacy of 84% to 94%, were spherical in form, and ranged in size from 425 to 485 nm. The study suggested that the F5 formulation had the best trapping efficiency and in-vitro release. In-vivo studies revealed that niosomal cream showed a better sustained anti-inflammatory effect than drug plain gel and the marketed product, which was confirmed by the paw edema test. Niosomal cream are promising formulations for sustained local delivery of Aceclofenac. It will provide a new method for future study. 

The Seabuckthorn oil (hippophae rhamnoides) is nutraceutical lipophilic drug. The reason for choosing this drug for the study lies in its abundant supply of polyunsaturated fatty acids (PUFA), antioxidants, flavonoids, phytosterols, carotenoids, and vitamins C, E, and K. These valuable components offer considerable benefits for treating a wide range of skin conditions. The literature review suggests that the melt-emulsification technique has been used for Seabuckthorn oil entrapped NLC. However, there have been no reports so far on the application of the microemulsion method for creating Seabuckthorn enriched NLCs. Hence an attempt was made to prepare Seabuckthorn oil entrapped NLC by microemulsion method. The aim of present research work was to formulate, evaluate and optimize the topical gel of Seabuckthorn oil enriched NLC. The preformulation studies were conducted on physical characteriscs (colour, odour, consistency), characterization tests (acid value, saponification value, peroxide value and iodine value), UV visible spectroscopy, FTIR spectroscopy of Seabuckthorn oil. In the current research, the preparation of NLCs involved conducting a screening of excipients, and the composition of these excipients was determined using a pseudoternary phase diagram. The Quality by Design (Qbd) principle was applied during the NLC preparation process, and a 32 factorial design was employed to optimize the formulation.The study aimed to achieve specific characteristics in NLCs, and to do so, the key parameters of total lipid content and Smix concentration were optimized using design expert software and assessed for % Entrapment efficiency, Particle Size, and Polydispersity Index.The optimal combination of 20% total lipid and 25% Smix resulted in NLCs with the minimum size of 308.9 nm, high entrapment efficiency (97.88%) and a narrow polydispersity index (0.320).The obtained optimized NLC formulation was subjected to various evaluations, including physical appearance, % drug loading capacity, viscosity, pH, zeta potential, and Scanning Electron Microscopy. Additionally, a gel version of the selected optimized NLC formulation was developed for topical application. The gel was then assessed for several parameters, including homogeneity, viscosity, pH, percent drug content, spreadability, extrudability and In vitro drug diffusion. From the results it was concluded that the Seabuckthorn oil was successfully loaded in to NLC by microemulsion technique and incorporated in to gel for topical purpose. 

The recent past has shown the increasing evidences indicating the role of traditional plants in the prevention of many diseases. The effectiveness of these medicinal plants mostly depends on the presence of several bioactive constituents that produces a raid action against many bacterial pathogens in a conventional application form. Also, due to the lesser side effects of these plants along with their therapeutic benefits, their usage has been increased thus serving as a crucial source of many ancient medicines. The present study was intended to evaluate the efficacy of the Citrus sinensis peel extracts against Streptococcus agalactiae isolated from pleural fluid. The analysis of the result for the antibacterial activity was found to be more effective with the methanolic extract with an inhibition zone of 14.0±0.1 mm than the ethyl acetate peel extract which showed a relatively less inhibition zone of 11.0±0.1mm respectively. As the methanolic extract of the plant showed good zone of inhibition against the test organism (Streptococcus spp.) therefore, it may be recommended that isolation of the bio-active components is needed for further research using animal model.