Abstract
Desvenlafaxine, an antidepressant in a group of drugs called selective serotonin and norepinephrine reuptake inhibitors (SNRI). A simple, precise and accurate High performance liquid chromatography method was developed and validated for the determination of Desvenlafaxine in bulk and tablet dosage forms. Chromatography was carried out by reversed phase technique on a C18 analytical column with a mobile phase composed of acetonitrile: aqueous Trifluoroacetic acid (0.1%) in the ratio of 70:30 v/v pumped at a flow rate of 1.0 ml/min. The detection was carried out at 225 nm at an ambient column temperature. The retention time was around 4.18 min. Calibration curves for Desvenlafaxine was linear over concentration range of 50 – 150 μg/ml with correlation coefficients 0.999. The mean recovery of Desvenlafaxine from different concentrations was 100%. The % RSD of precision was found to be 0.32%. The method was successfully validated in accordance to ICH guidelines. It was observed that excipient present in formulation did not interfere with peak of Desvenlafaxine. In the conclusion, the developed and validated method was successfully employed for the routine quality control analysis of bulk and pharmaceutical formulations and can be applied for bioequivalence study of Desvenlafaxine.
