The present study aimed to isolate endophytic fungus Absidia species from Nyctanthes arbor-tristis Linn. the methanolic extracts of endophytic fungus Absidia was subjected to characterisation through UV-Visible spectrophotometry (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR) and Gas chromatography - Mass spectrometry (GC-MS) analytical techniques. The fungal extract was scanned in the wavelength ranging from 190 – 1100 nm using UV-Vis Spectrometer, which exhibited characteristic peaks with distinct absorption that were detected. These peaks were identified in near infrared region that is above 800 nm, which poses difficulty in allotting absorption peak to any particular component. Therefore, UV-Vis analysis is supported with GC-MS and FTIR for confirmation of presence of secondary metabolites.  The FTIR spectrum validates the presence of alcohols, phenols, alkenes, alkynes, aromatics, carboxylic acids, nitro compounds, alkyl halides, aliphatic amines, primary and secondary amines. These functional groups possess medicinal properties. The GC-MS analysis of methanolic extracts of Absidia from N. arbor-tristis displays several peaks, determining the existence of 28 bioactive compounds. The result authenticates the presence of bioactive compounds in the extracts of Absidia, that might play important role as curative of various diseases and as an anticancer agent.However further investigations are required to evaluate their potential therapeutic effects.

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