Ramipril,
a long-acting angiotensin-converting enzyme (ACE) inhibitor, is widely used for
the treatment of hypertension, congestive heart failure, and for the prevention
of cardiovascular events. Given its clinical importance, the development of
reliable analytical methods for the quantification of Ramipril in
pharmaceutical formulations and biological matrices is crucial. This review
compiles and critically examines various validated analytical techniques
employed for the determination of Ramipril, both as a single agent and in
combination with other drugs. The methodologies reviewed include UV
spectrophotometry, high-performance liquid chromatography (HPLC),
high-performance thin-layer chromatography (HPTLC), liquid chromatography-mass
spectrometry (LC-MS), and fluorimetric techniques. Each method is evaluated in
terms of sensitivity, specificity, accuracy, precision, and applicability to
different pharmaceutical dosage forms. The review highlights the importance of
selecting suitable analytical approaches for routine quality control,
pharmacokinetic studies, and stability testing of Ramipril, thereby
contributing to improved therapeutic monitoring and regulatory compliance.
