Temple flower waste constitutes a significant portion of organic refuse, with commonly discarded flowers such as rose, marigold, and hibiscus often left in open areas or water bodies, contributing to environmental pollution. Notably, certain floral species—particularly Hibiscus abelmoschus—possess bioactive compounds with known antimicrobial potential. This study aimed to formulate and evaluate a topical cream incorporating Hibiscus abelmoschus seed oil extract and assess its antimicrobial efficacy. Oil was extracted from Hibiscus abelmoschus seeds using petroleum ether. A series of cream formulations were developed using standard excipients, including borax, beeswax, white soft paraffin, and preservatives such as methyl paraben and propyl paraben. Formulations were assessed for physicochemical parameters including pH, viscosity, spreadability, and extrudability. The antimicrobial activity of various Hibiscus rosa-sinensis extracts (hexane, ethyl acetate, methanol, and aqueous) was evaluated against Staphylococcus aureus using the agar diffusion method. Minimum inhibitory concentration (MIC) values were determined for the ethyl acetate extract. Among the tested extracts, the ethyl acetate and methanolic fractions demonstrated significant antimicrobial activity, while the hexane extract exhibited minimal efficacy. The ethyl acetate extract showed the lowest MIC against Staphylococcus aureus, suggesting a strong inhibitory effect. The results highlight the potential of Hibiscus abelmoschus seed oil, particularly in ethyl acetate and methanol extracts, as a promising antimicrobial agent for topical application. This approach not only utilizes biodegradable floral waste but also offers a sustainable alternative for developing plant-based dermatological therapeutics.