India is the country having rich source of medicinal plants used in Indian System of Medicine which include Ayurveda, Siddha, and Unani in additional to traditional medicinal practices. Secondary metabolites of plant products have greater advantages in pharmacological activities with minimal side effect. Gymnema tingens (Roxb) Wt. & Arn. is one of the endangered medicinal plants of the genus Gymnema belonging to family Asclepiadaceae. It is used in treating the diabetes traditionally. There is a meager scientific data regarding the standardization of this herbal drug. So made an attempt to standardize the botanical through pharmacognostical approach of    microscopy, organoleptic study, powder microscopy, physicochemical parameter, phytochemical analysis and fluorescence analysis. Results shows a unique feature like multicellular uniseriate trichomes, druses of calcium oxalate crystal, paracytic stomata, specific characteristic mild odour, bulk density is 0.38g/cc, ash value is 8.5%, specific gravity is 1.24g /cm³, pH values is 5.6 and aqueous extractive is 3 %. Presence of alkaloid, phytosterol, saponin, flavonoid and tannins. Fluorescence analysis of a drug with different chemical reagent under different wavelength emits a particular colour due to the presence of a particular phytochemical composition. Drug powder with acetone under long wavelength emits saffron colour. The study conclude that the results obtained could be utilized as a reference for setting limits for the reference standards of Gymnema tingens botanical to check the quality and purity of the herbal drug. This data can be used by the scientific community to develop a different formulation   to serve the society locally to globally.

1.      Elekofehinti O O. Saponins: Anti-diabetic principles from medicinal plants – A review. Pathophysiology. 2015; 22(2):95–103.

2.      Liu MY, Zhou T X, Zhang J Y, Liao GF, Lu R M, Yang X Z. Identification of C21 Steroidal Glycosides from Gymnema sylvestre (Retz.) and Evaluation of Their Glucose Uptake Activities. Molecules. 2021; 26 (21):2-14.

3.      Fabio G D, Romanucci V, Marco AD, Zarrelli A. Triterpenoids from Gymnema sylvestre and Their Pharmacological Activities. Molecules. 2014; 19(8):10956–10981.

4.      Sahu N P, Mahato S B, Sarkar SK, Poddar G. Triterpenoid saponins from Gymnema sylvestre Phytochemistry. 1996; 41(4):1181–1185.

5.      Yoshikawa K, Matsuchika K, Takahashi K, Tanaka M, Arihara S, Chang H C, Wang J D. Pregnane Glygosides, Gymnepregosides G-Q from the Roots of Gymnema alternifolium. Chem. Pharm. Bull. 1999; 47:798–804.

6.      Liu X, Ye WC, Yu B, Zhao SX, Wu H M, Che CT. Two new flavonol glycosides from Gymnema sylvestre and Euphorbia ebracteolate. Carbohydrate Research. 2004; 339(4):891–895.

7.      Kamei K, Takano R, Miyasaka A, Imoto T, Hara S J. Amino Acid Sequence of Sweet-Taste-Suppressing Peptide (Gurmarin) from the Leaves of Gymnema sylvestre. The Journal of Biochemistry.1992; 111(1):109–112.

8.      Wu X, Mao G, Fan Q, Zhao T, Zhao T, Li F. et al. Isolation, purification, immunological and anti-tumor activities of polysaccharides from Gymnema sylvestre.  Food Res. J. 2012; 48(2):935-939.

9.      Vats S, Kamal R. In vivo and in vitro evaluation of sterols from Gymnema sylvestrte R. Br. Pak. J. Biol. Sci. 2013; 16(23):1771–1775. [PubMed] [Google Scholar]

10.   Nagella P, Chung I M, Murthy H N. In vitro production of gymnemic acid from cell suspension cultures of Gymnema sylvestre R. Br.   Eng. Life. Sc. 2011; 11(5):537-540.

11.   Diwan P V, Margaret I, Ramakrishna S. Influence of Gymnema sylvestre on inflammation.  Inflammopharmacology. 1995; 3:271-277.

12.   Sinha S N, Saha G C, Biswas M. Screening of Various Solvent Extracts of Gymnema sylvestre R.Br. Leaf for Antibacterial Activity.  Adv. Biores. 2010; 1(2):25-28

13.   Malik J K, Manvi F V, Nanjwade B R, Dwivedi D K, Purohit P, Chouhan S. Anti-arthritic activity of leaves of Gymnema sylvestre R.Br. Pharm. Lett. 2010; 2:336-341.

14.   Daisy P, Eliza J, Farook K A M. J A. Novel dihydroxy gymnemic triacetate isolated from Gymnema sylvestre possessing normoglycemic and hypolipidemic activity on STZ-induced diabetic rats. J. Ethnopharmacol. 2009; 126(2):339–344.

15.   Ramkumar K M, Manjula C, Elango B, Krishnamurthi K, Devi S S, Rajaguru P. In vitro cytotoxicity of Gymnema montanum in human leukaemia HL‐60 cells; induction of apoptosis by mitochondrial membrane potential collapse. Cell Proliferation. 2013; 46:263-271.

16.   Kumar S, Gupta P, Sharma S, Kumar D.A review on immunostimulatory Plants Chin. J. Integr. Med. 2011; 9(2):117–128.]

17.   Kahksha HJ H, Alam O, Naaz S, Sharma V, Manaithiya A, Khan J, et al. Ethnopharmacol. 2020; 286:114908.

18.   Chattopadhyay RR. A comparative evaluation of some blood sugar lowering agents of plant origin. Journal of Ethnopharmacology. 1999; 67(3): 367-372.

19.   Ota M, Shimizu Y, Tonosaki K, Ariyoshi Y. Chemical studies of gurmarin- a sweetness suppressing polypeptide. Research Developments in Agricultural and Biological Chemistry. 1998; 2(2): 445-453.

20.   Shimizu K, Iino A,  Nakajima J, Tanaka K, Nakajyo S, Urakawa, N, et al. Suppression of glucose absorption by some fractions extracted from Gymnema sylvestre leaves. Journal of Veterinary Medical Science. 1997; 59(4): 245-251.

21.   Sumy Oommen, Ved DK, Krishnan R. Tropical Indian Medicinal Plants Propagation methods. Bangalore: FRLHT; 2000.

22.   Jiang Y, Li B T. Flora of China. Beijing. Vol 63.  Science Press; 1977.pp. 422 [Google Scholar]

23.   Khare D P, Khare A, Khare M P. Carbohydr. Res. 1980; 81:2 85–294. [Google Scholar]

24.   He J C, Wang M Y, Dong Z C. Nat. Prod. Res. Dev. 2013; 25:1673–1675. [Google Scholar]

25.   Tian J. Studies on Chemical Constituents of Gymnema tingens, Peking Union Medical College, 2013 [Google Scholar]

26.   He J, Tang P, Liu M, Liao G, Lu R, Yang X. Triterpenoid saponins and C21 steroidal glycosides from Gymnema tingens and their glucose uptake activities. RSC Adv. 2023; 13(11):7503-7513.

27.   Tian J, Ma QG, Yang JB, Wang AG, Ji TF, Wang YG, et al. Hepatoprotective phenolic glycosides from Gymnema tingens. Planta Med. 2013; 79(9):761-767.

28.   Ramaswamy S N, Radhakrishna Rao M, Govindappa D A. Flora of Shimoga District, Karnataka. 1 st ed. Mysore: Prasaranga University of Mysore; 2001.

29.   Gamble J S, The flora of the Presidency of Madras. Rep. ed. Vol 2. Dehra Dun:

30.   Bishen Singh Mahendras Pal Singh; 2011.

31.   Iyangar M A, Nayak S G K. Anatomy of crude drugs. 8th ed. K M.C Manipal: Published by Prof. Iyengar M A., College of Pharmaceutical Sciences; 2001.

32.   Khandelwal K R. Practical Pharmacognosy.18th ed. Pune: Nirali Publication;    2007. pp. 146-148.

33.   Govt. of India. The Ayurvedic Pharmacopoeia of India. Part I. Vol IV.1st ed. New Delhi: Department of Indian System of Medicine and Homoeopathy; 2004. pp. 158-176.

34.   Lachman L, Liberman HA, Kanig JL. The Theory and Practice of Industrial Pharmacy.3_rd ed. Mumbai: Varghese Publishing House; 1991. pp.67.

35.   Kokate C K, Purohit A P, Gokhale S B.  Pharmacognosy. 33rd ed. Pune: Nirali Prakashan; 2005. pp. 593-597.

36.   Chase C R., Pratt R J.  Fluorescence Analysis of Powdered drugs with particular reference to development of system identification.  J. M. Pharm. Assoc. 1949; 38:324-331.