Abstract
A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Elvitegravir (EVG) in tablet dosage forms. An Inertsil ODS 3V, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.03M KH2PO4 in water adjusting the pH-3.2 with dilute O-Phosphoric Acid mixed in Methanol & Water in ratio of 80:20 v/v & Acetonitrile & Buffer in ratio of 60:40 v/v, was used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 257 nm. The retention time was 6.250 min and the detector response was linear in the concentration range of 80-960 µg/mL for EVG successively. The respective linear regression equation being Y= 9474.289x + 147734.8116 for EVG. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.4 & 1.2 µg for EVG. The percentage assay of EVG was 98.60%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of EVG in bulk drug and in its pharmaceutical dosage forms.