Ofloxacin is one of a new generation of fluorinated quinolones structurally related to nalidixic acid. It is orally administrated broad spectrum anti-bacterial & some anaerobes. Moreover ofloxacin achieves high concentration in most tissues and body fluids. The result of clinical trials with ofloxacin have confirmed the potential for use in a wide range of infections, which was indicated by its in vitro anti-bacterial & pharmacokinetics profiles. It has proven effective against a high percentage of infection caused by gram-negative organisms, slightly less effective against gram positive infections, & effective against some anaerobic infections. Ofloxacin is well tolerated & although experience with the drug in clinical practices to date is limited, bacterial resistance does not appear to develop readily. Thus, ofloxacin is an orally active drug which offers a valuable alternative to other broad spectrum anti-bacterial drug. The synythesis of Ofloxacin was carried out in this article. And quinoline-4(1H)-one is the intermediate that was found in this Ofloxacin. There physiochemical properties & characterization of impurity is also described.