The plant Ageratum conyzoides is widely used in folk medicine in the Villages in west UP and Uttarakhand, India. The aim of this study is evaluate the anti bacterial study of the n-hexane, Chloroform, ethyl acetate, methanol and water extract of the Ageratum conyzoides (Linn.) leaves belonging to family Asteraceae were investigated against Two Gram positive bacteria like Staphylococcus aureus and streptococcus pneumoniae as well as Three Gram negative bacteria like Escherichia coli, Pseudomonas aeruginosa and klebisella pneumoniae. Because of such concerns, the necessity to find potentially fruitful, healthy safer and natural alternative preservatives is increased. Plant extracts have been used to control food poisoning defect and preserve foodstuff. Agar disc diffusion techniques were used for in-vitro antibacterial Screening. Methanolic extract was potentially effective with variable efficiency against the tasted four bacterial stains Staphylococcus aureus, streptococcus pneumoniae, Pseudomonas aeruginosa and klebisella pneumoniae at concentration of 70 mg/m. Methanolic extract was the most effective plant extract and showed bacteriostatic and bactericidal activities against the highly susceptible strains of food borne pathogenic bacteria with MIC’s ranged from 16.0 to 32.0 mg/ml. Antibacterial activity of total five bacteria were used for evaluation of n-hexane chloroform, ethyl acetate, methanol and water extracts of Ageratum conyzoides. Result of zone of inhibition showed in both tables. Water extract negative test for all five bacterial strain. Antibacterial activity was measured by noting zone of inhibition in disc diffusion. Minimum inhibitory concentration was also noted. Methanolic extract of Ageratum conyzoides for use as antibacterial drug.