The objective of the study is to assess the anti-obesity activity of ayurvedic and homeopathic formulations namely Stholyantak churna and Phytolacca berry in high fat cafeteria diet induced obesity (HFCD). Obesity was induced by administration of HFCD for a period of 42 days and both the formulations were given for 42 days. Administration of HFCD significantly increased the body weight and organ weights. Hepatic marker enzymes such as ALT, ALP and AST levels were increased and HDL level was decreased in HFCD control group compared to normal group. Treatment with formulations showed a significant reduction in the body weight gain, organ weight of the liver, kidney, spleen. Levels of hepatic enzymes and lipid profile were restored. The total phenolic content of the Phytolacca berry and Stholyantak churna was found to be 31.16?g/g and 70.39 ?g/g. The total flavonoid content of the Phytolacca berry and Stholyantak churna was found to be 12.28?g/g and 41.36?g/g. Chemical investigation of Phytolacca berry and Stholyantak churna led to identify the flavonoids quercetin and phenols gallic acid were present in formulations by HPTLC technique along with standard markers. The HPTLC fingerprinting of formulations revealed the presence of the 0.04% of gallic acid in Phytolacca berry, Stholyantak churna in aqueous showed 0.47% of gallic acid and Stholyantak churna in methanol showed 0.08% of quercetin and 0.03% of gallic acid. Based on the above results, it was concluded that the ayurvedic and homeopathic formulations, Stholyantak churna and Phytolacca berry possess significant anti-obesity activity in HFCD induced obesity model. Stholyantak churna possess higher anti-obesity activity than Phytolacca berry.
