The aim of present study was to evaluate anthelmintic potential of the juice of Euphorbia ligularia using Pheretima posthuma and Ascardia galli as test worms. Various concentrations (10 100 mg/ml) of juice were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate (10 mg/ml) was included as standard reference and distilled water as control. The results of present study indicated that juice significantly demonstrated paralysis, and also caused death of worms especially at higher concentration of 100 mg/ml, as compared to standard reference Piperazine citrate. In conclusion, the E. ligularia juice as an anthelmintic have been confirmed.
