The main purpose of this study was to find out the antibacterial activity of individual crude extract and fraction and mixture active fraction of Camellia sinensis. For antibacterial test, disc diffusion technique was used against human pathogenic bacterial strains. The noteworthy inhibition of ethanol mixed methanol active fraction was inhibit 7 mm zone against Streptococcus pneumoniae, and 8 mm zone against Staphylococcus aureus, it was higher activity than allindividual fractions and crude extract. In the GC-MS analysis, 20 bioactive phytochemical compounds were identified in the ethanolic fraction of Camellia sinensis. Further work needs to be done in these materials including fractionation to isolate active constituents and subsequent pharmacological evaluation.