Abstract
Consistent evidence suggested that copper is an important environmental pollutants which can be easily found in all kinds of foodstuffs. Copper can induce toxicity to the liver cells and intern it affects the genetic component of the human body. Total number of broiler consumer has been increased tremendously in recent decades, so the present study mainly focused on the ability of Copper Sulphate (CuSo4) to affect total genomic DNA content, Antioxidant activity, and total protein content of broiler’s liver cells. The experiment was carried out in to three fundamental steps after the In vitro CuSO4 exposure to the Broiler’s liver cells. First, we evaluated the percentage of Antioxidant Activity (scavenging activity) reduction by DDPH method. Secondly, DNA status was evaluated by DNA fragmentation and finally total protein quantity reduction was estimated by Lowry’s method. Further studies should be carried out to better investigate the actual apoptosis mechanism and to further characterize the nature of DNA damage. The data obtained are important for the analysis of genetic and health risks produced by CuSO4 in liver cells.