Abstract
A new simple, selective, rapid, precise reversed phase high performance liquid chromatography method has been developed and validated for the simultaneous estimation of Piracetam and Citicoline in a pharmaceutical dosage form. The separation was made using Inertsil C18, (250 x 4.6) mm, 5μm column. Mobile phase used contained Phosphate buffer and acetonitrile in gradient mode at wavelength of 210nm. The mobile-phase flow rate and the sample volume injected were 1 ml/min and 10 μl, respectively. Retention time of Citicoline and Piracetam were found to be 4.2 ±0.2mins, 12.3±0.2minsrespectively. A good linear relationship (Citicoline r=0.999& Piracetam r=0.999) was observed over a concentration range of 80.50 to 603.73 μg/ml of Piracetam and 50.66 to 379.94 μg/ml of Citicoline. The limit of detection (LOD) and limit of quantification (LOQ) for Citicoline was found to be 0.08 ppm&0.26ppm and for Piracetam it was found to be0.12 ppm & 0.42ppm. It was concluded that in the present developed RP- HPLC method, the standard and sample preparation required less time and without tedious extraction. The developed method is simple, rapid, and accurate, hence can be used for routine quality control analysis in Pharmaceutical industry. The method was optimized on gradient mode; offering better resolution of peak of interest as well as impurities in contrast to all existing methods which are on isocratic mode.