Abstract
Urinary tract infection is a serious health threat with respect to antibiotic resistance and biofilm formation being the prime cause for the antibiotic resistance, the present study aimed to detect biofilm formation in Multidrug-resistant uropathogenic Klebsiella pnemoniae isolates by using Tissue Culture Plate Method, Tube adherence method and Modified Congo Red Agar Method. The study was conducted in the department of Microbiology of Maharishi Markandeshwar institute of medical science and research, Mullana .Out of total 550 samples, 23.2%(128) was uropathogenic. Out of 128 samples 60 samples were Klebsiella pnemoniae strains. They were multidrug resistant (cephalosporins and floroquinlones). The rate of biofilm producers is detected by three methods which were 38(63.33%). In which, Modified Congo Red Agar Method was best 36(60%) followed by Tube adherence method 33 (55%) and Tissue Culture Plate Method 28 (46.67%).In present study, higher biofilm producing isolates were detected in indoor and urban patients 76.31%(29) and 57.89 %(22) respectively. Furthermore, regarding the age highest percentage of biofilm producing isolates was found between 50-79 years (55.26%) followed by 20-49 years of age (23.68%). Dominancy of female (71.05%) were established over the male of only (28.94%). The data on antibiogram revealed that Imipenem (73.68%) followed by Amikacin (68.42%) and Nitrofurantoin(52.63%) were most effective antibiotic for biofilm producing Multidrug-resistance Klebsiella pneumoniae .So at last we can conclude that the urine isolates specially Multidrug-resistance Klebsiella pneumoniae should be screened for biofilm production and should put for antibiotic sensitivity to determine antibiotic policy in the hospital.