Abstract
The present work involves development and validation of a simple, sensitive and specific Reverse Phase-High Performance Liquid Chromatographic (RP-HPLC) method for the estimation of Nebivolol and Valsartan in Bulk drug and Pharmaceutical dosage form to provide an economical and easy method than the existing methods. The chromatoghraphic run followed an isocratic mode utilizing an INTERSIL C18 column (250*4.6mm and 5 μm) with mobile phase buffer (pH 2.5): methanol: Acetonitrile in the ratio of 30:20:50 at a flow rate of 1.0ml / min. The chromatographic detection was monitored at 210 nm. The retention times for Nebivolol & Valsartan were found to be 2.523 & 4.417 respectively. The method was validated as per ICH guidelines for system suitability, linearity, precision, accuracy, specificity, robustness, LOD and LOQ. The method was linear with r2 values 0.998 & 0.999 for nebivolol and valsartan respectively. The accuracy of the method was evaluated at three levels i.e. 80%, 100% & 120% and the recoveries were found to be between 98.6 - 101.3 %. The LOD and LOQ for estimation of Nebivolol& valsartan are found to be 0.0121μg / ml, 0.0365μg / ml, and 3.154μg / ml, 9.557μg / ml respectively. Proposed method can be successfully applied for the quantitative determination of Nebivolol and Valsartan in Bulk drug and Pharmaceutical dosage form.
