Abstract

Diabetes causes increased oxidative stress, which is thought to play an important role in the pathogenesis of various diabetic complications. Commiphora mukul (CM) gum resin ethanoilc extract has several biological properties including hypolipidemic, amtidabetic and antioxidant activity. This study was undertaken to evaluate the effects of CM gum resin ethanolic extract on polyol path way enzymes and intestinde disaccharidases in streptozotocin (STZ) induced diabetic and high fructose diet (HFD) induced insulin resistant rats. The male Wistar albino rats were randomly divided into six groups of eight animals each: two of these groups (groups C and C+CM) were fed with standard pellet diet and the other two groups (groups F and F+CM) were fed with high fructose diet (HFD) (66 %), other two groups (D group and D+CM) were induced by intraperitoneal injection of streptozotocin (STZ, 55 mg/kg body wt) to male Wistar rats. C. mukul gum resin ethanolic extract in water (200 mg/kg body weight/day) was administered orally to group C+CM, D+CM and group F+CM. At the end of 60-day experimental period biochemical parameters related to Polyol pathway enzymes like liver, pancreas and heart tissues of aldose reductase (AR) and sorbital dehydrogenase (SDH) and intestine disccharidases enzymes like maltase, sucrose and lactase enzymes were performed. The STZ induced diabetic and fructose fed insulin resistant rats showed increased levels of enzymatic activities aldose reductase (AR) and sorbital dehydrogenase (SDH) and intestine disccharidases enzymes like maltase, sucrose and lactase enzymes. Administration of C.mukul (200 mg/kg bw) to STZ induced and fructose fed insulin resistant rats for 60 days significantly reversed the above parameters towards normally. In the present study the enhanced or elevated activities of polyol pathway enzymes and intestinal disaccharidases of insulin deficient diabetic and insulin resistant rats were prevented by the C. mukul treatment. It may also contribute to intestinal disaccharidase inhibitory activity; another beneficial property of C. mukul in decreasing the polyol pathway towards preventing the oxidative stress induced diabetic complications, and hence this plant can be used as an adjuvant for the prevention and management of insulin resistance and insulin deficient to it.