IN-VITRO SCREENING OF METHANOLIC LEAF EXTRACTS OF ALBAZIA SAMAN (Jacq.)Merr, FOR THEIR CYTOTOXIC ACTIVITY
Suresh Arumugam*, Anju Venugopal, Suriya Annamalai and Senthil kumar Natesan
Department of Pharmacology,JKK Munirajah Medical Research Foundation College of Pharmacy, B.Komarapalayam, Namakkal (Dist), Tamilnadu.
The present study was designed to evaluate the invitro cytotoxic potential of methanolic extract of Albazia saman leaves. The extraction of leaves was carried out by Soxhlet apparatus using methanol. The Cytotoxicity of methanolic extract was tested by Brine shrimp lethality assay, Trypan blue dye exclusion method, Neutral red Cytotoxicity assay, MTT assay and Ethidium bromide – acridine orange staining. Studies have shown differential sensitivities to several natural compounds between tumor and normal cells in vitro and the results obtained from the present study show that the methanol extract from Albazia saman leaves to DLA cell lines, and L929 cell lines. Moreover it has protective effect against cancer by their effect on signal transduction in cell proliferation and angiogenesis. It also justifies the folklore medicinal uses and claims about the therapeutic values of this plant as curative agent against tumor and we therefore, suggest further, the purification and characterization of the phytochemicals along with investigations needed to provide some additional insight into the in vivo cytotoxicity activity of the plants with a view to obtaining useful chemotherapeutic agent. Hence the leaf extract is proved the herbal use of cytotoxic activity and it is recommended as anti-cancer drug further to isolate the bioactive compound and drug designing.